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En tiempo real, semi-automatizado de medición fluorescente de la superficie de las vías respiratorias pH líquido de las células epiteliales de las vías respiratorias humanas primarias
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10:18 min
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June 13, 2019
DOI:
1Epithelial Research Group, Institute for Cell and Molecular Biosciences, Faculty of Medical Sciences,Newcastle University, 2Respiratory Group, Institute of Cellular Medicine, Faculty of Medical Sciences,Newcastle University, 3Paediatric Respiratory Medicine, Great North Children’s Hospital,Newcastle upon Tyne Hospitals NHS Foundation Trust, 4Boehringer Ingelheim Pharma GmbH & Co
Summary
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Presentamos un protocolo para realizar mediciones dinámicas del pH líquido de la superficie de las vías respiratorias en condiciones de película delgada utilizando un lector de placas.
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Cite this Article
Saint-Criq, V., Haq, I. J., Gardner, A. I., Garnett, J. P., Ward, C., Brodlie, M., Gray, M. A. Real-Time, Semi-Automated Fluorescent Measurement of the Airway Surface Liquid pH of Primary Human Airway Epithelial Cells. J. Vis. Exp. (148), e59815, doi:10.3791/59815 (2019).
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