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Examining the Dynamics of Cellular Adhesion and Spreading of Epithelial Cells on Fibronectin During Oxidative Stress
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Biochemistry
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JoVE Journal Biochemistry
Examining the Dynamics of Cellular Adhesion and Spreading of Epithelial Cells on Fibronectin During Oxidative Stress

Examining the Dynamics of Cellular Adhesion and Spreading of Epithelial Cells on Fibronectin During Oxidative Stress

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7,271 Views

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10:57 min

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October 13, 2019

DOI:

10.3791/59989-v

DOI:
10.3791/59989-v

•

10:57 min
October 13, 2019

•

3 Views
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1Department of Cell Biology and Physiology,University of North Carolina at Chapel Hill, 2Department of Chemistry,High Point University, 3Biotechnology Program and the Department of Molecular Biomedical Sciences,North Carolina State University

Summary

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This method is useful for quantifying the early dynamics of cellular adhesion and spreading of anchorage-dependent cells onto the fibronectin. Furthermore, this assay can be used to investigate the effects of altered redox homeostasis on cell spreading and/or cell adhesion-related intracellular signaling pathways.

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Tolbert, C. E., Palmquist, L., Dixon, H. L., Srougi, M. C. Examining the Dynamics of Cellular Adhesion and Spreading of Epithelial Cells on Fibronectin During Oxidative Stress. J. Vis. Exp. (152), e59989, doi:10.3791/59989 (2019).

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