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尿道感染小鼠模型产生的单细胞内细菌群落的分离, 用于下游单细胞分析
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation of Single Intracellular Bacterial Communities Generated from a Murine Model of Urinary Tract Infection for Downstream Single-cell Analysis
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尿道感染小鼠模型产生的单细胞内细菌群落的分离, 用于下游单细胞分析

DOI:
10.3791/58829-v

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07:34 min

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April 16, 2019

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, , , , ,
1Infectious Diseases Group,Genome Institute of Singapore, 2Department of Chemical and Biomolecular Engineering,National University of Singapore, 3Division of Infectious Diseases, Department of Medicine, Yong Loo Lin School of Medicine,National University of Singapore

Chapters

  • 00:04Title
  • 00:41Glass Capillary Preparation
  • 02:06Bladder Epithelial Cell Harvest
  • 03:53Intracellular Bacterial Community (IBC) Isolation
  • 05:18Results: Harvested IBC Are Pure and Can Be Used for Downstream Analysis
  • 06:30Conclusion

Summary

Automatic Translation

Automatic Translation

该方案描述了一种从尿路感染的小鼠模型中分离单个感染膀胱上皮细胞的简单方法。

Tags

Intracellular Bacterial CommunitiesIBCsUrinary Tract InfectionUTIMouse BladderMouth MicropipettingGlass Capillary TubeBladder Epithelial CellsSingle-cell Analysis

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