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Aislamiento de las comunidades bacterianas intracelulares solo generados a partir de un modelo murino de infección del tracto urinario para posteriores análisis unicelular
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation of Single Intracellular Bacterial Communities Generated from a Murine Model of Urinary Tract Infection for Downstream Single-cell Analysis
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Aislamiento de las comunidades bacterianas intracelulares solo generados a partir de un modelo murino de infección del tracto urinario para posteriores análisis unicelular

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7,734 Views

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07:34 min

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April 16, 2019

DOI:

10.3791/58829-v

DOI:
10.3791/58829-v

•

07:34 min
April 16, 2019

•

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, , , , ,
1Infectious Diseases Group,Genome Institute of Singapore, 2Department of Chemical and Biomolecular Engineering,National University of Singapore, 3Division of Infectious Diseases, Department of Medicine, Yong Loo Lin School of Medicine,National University of Singapore

Summary

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Este protocolo describe un método sencillo de aislar a las células epiteliales individuales, vejiga infectada de un modelo murino de infección del tracto urinario.

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Yang, E., Chee, J. L., Duraiswamy, S., Chen, S., Lees, K., Chen, S. L. Isolation of Single Intracellular Bacterial Communities Generated from a Murine Model of Urinary Tract Infection for Downstream Single-cell Analysis. J. Vis. Exp. (146), e58829, doi:10.3791/58829 (2019).

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