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Isolement des simples communautés bactériennes intracellulaires généré à partir d’un modèle murin d’Infection des voies urinaires pour l’analyse de cellules individuelles en aval
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation of Single Intracellular Bacterial Communities Generated from a Murine Model of Urinary Tract Infection for Downstream Single-cell Analysis
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Isolement des simples communautés bactériennes intracellulaires généré à partir d’un modèle murin d’Infection des voies urinaires pour l’analyse de cellules individuelles en aval

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7,734 Views

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07:34 min

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April 16, 2019

DOI:

10.3791/58829-v

DOI:
10.3791/58829-v

•

07:34 min
April 16, 2019

•

2 Views
, , , , ,
1Infectious Diseases Group,Genome Institute of Singapore, 2Department of Chemical and Biomolecular Engineering,National University of Singapore, 3Division of Infectious Diseases, Department of Medicine, Yong Loo Lin School of Medicine,National University of Singapore

Summary

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Ce protocole décrit une méthode simple permettant d’isoler les cellules épithéliales unique, infectés par la vessie d’un modèle murin d’infection urinaire.

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Cite this Article

Yang, E., Chee, J. L., Duraiswamy, S., Chen, S., Lees, K., Chen, S. L. Isolation of Single Intracellular Bacterial Communities Generated from a Murine Model of Urinary Tract Infection for Downstream Single-cell Analysis. J. Vis. Exp. (146), e58829, doi:10.3791/58829 (2019).

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