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Isolation of Single Intracellular Bacterial Communities Generated from a Murine Model of Urinary Tract Infection for Downstream Single-cell Analysis
JoVE 杂志
免疫与感染
This content is Free Access.
JoVE 杂志 免疫与感染
Isolation of Single Intracellular Bacterial Communities Generated from a Murine Model of Urinary Tract Infection for Downstream Single-cell Analysis

Isolation of Single Intracellular Bacterial Communities Generated from a Murine Model of Urinary Tract Infection for Downstream Single-cell Analysis

DOI:
10.3791/58829-v

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07:34 min

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April 16, 2019

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, , , , ,
1Infectious Diseases Group,Genome Institute of Singapore, 2Department of Chemical and Biomolecular Engineering,National University of Singapore, 3Division of Infectious Diseases, Department of Medicine, Yong Loo Lin School of Medicine,National University of Singapore

Chapters

  • 00:04Title
  • 00:41Glass Capillary Preparation
  • 02:06Bladder Epithelial Cell Harvest
  • 03:53Intracellular Bacterial Community (IBC) Isolation
  • 05:18Results: Harvested IBC Are Pure and Can Be Used for Downstream Analysis
  • 06:30Conclusion

Summary

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This protocol describes a simple method of isolating single, infected-bladder epithelial cells from a murine model of urinary tract infection.

Tags

Keywords: Intracellular Bacterial CommunitiesIBCsUrinary Tract InfectionUTIMouse BladderMouth MicropipettingGlass Capillary TubeBladder Epithelial CellsSingle-cell Analysis

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