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下流の単一細胞分析のため尿路感染症のマウスモデルから生成された単一細胞内細菌群集の分離
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation of Single Intracellular Bacterial Communities Generated from a Murine Model of Urinary Tract Infection for Downstream Single-cell Analysis
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下流の単一細胞分析のため尿路感染症のマウスモデルから生成された単一細胞内細菌群集の分離

日本語

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7,734 Views

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07:34 min

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April 16, 2019

DOI:

10.3791/58829-v

DOI:
10.3791/58829-v

•

07:34 min
April 16, 2019

•

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, , , , ,
1Infectious Diseases Group,Genome Institute of Singapore, 2Department of Chemical and Biomolecular Engineering,National University of Singapore, 3Division of Infectious Diseases, Department of Medicine, Yong Loo Lin School of Medicine,National University of Singapore

Summary

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このプロトコルでは、単純な尿路感染症のマウスモデルからの単一、感染して膀胱の上皮細胞を分離する方法について説明します。

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Yang, E., Chee, J. L., Duraiswamy, S., Chen, S., Lees, K., Chen, S. L. Isolation of Single Intracellular Bacterial Communities Generated from a Murine Model of Urinary Tract Infection for Downstream Single-cell Analysis. J. Vis. Exp. (146), e58829, doi:10.3791/58829 (2019).

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