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Isolation of Single Intracellular Bacterial Communities Generated from a Murine Model of Urinary Tract Infection for Downstream Single-cell Analysis
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Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Isolation of Single Intracellular Bacterial Communities Generated from a Murine Model of Urinary Tract Infection for Downstream Single-cell Analysis

Isolation of Single Intracellular Bacterial Communities Generated from a Murine Model of Urinary Tract Infection for Downstream Single-cell Analysis

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07:34 min

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April 16, 2019

DOI:

10.3791/58829-v

DOI:
10.3791/58829-v

•

07:34 min
April 16, 2019

•

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, , , , ,
1Infectious Diseases Group,Genome Institute of Singapore, 2Department of Chemical and Biomolecular Engineering,National University of Singapore, 3Division of Infectious Diseases, Department of Medicine, Yong Loo Lin School of Medicine,National University of Singapore

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This protocol describes a simple method of isolating single, infected-bladder epithelial cells from a murine model of urinary tract infection.

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Intracellular Bacterial CommunitiesIBCsUrinary Tract InfectionUTIMouse BladderMouth MicropipettingGlass Capillary TubeBladder Epithelial CellsSingle-cell Analysis

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Yang, E., Chee, J. L., Duraiswamy, S., Chen, S., Lees, K., Chen, S. L. Isolation of Single Intracellular Bacterial Communities Generated from a Murine Model of Urinary Tract Infection for Downstream Single-cell Analysis. J. Vis. Exp. (146), e58829, doi:10.3791/58829 (2019).

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