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09:24 min
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August 05, 2010
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This video will demonstrate methods to transurethrally induce mouse urinary tract infections and quantify the extent of resulting infections.
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Transurethral InductionMouse Urinary Tract InfectionUropathogenic Bacterial StrainsAgarUropathogenic E. ColiLuria-Bertani AgarColony MorphologyCulture In BrothLB BrothType I PiliVirulence FactorPhosphate Buffered SalineFemale MiceIsoflurane AnesthesiaTransurethral InoculationPolyethylene Tubing-covered SyringesInocula ConcentrationCfu Per MouseUrine SamplesBladderKidneysHarvesting OrgansMinced OrgansHomogenizationBlue Bullet HomogenizerSerial DilutionColony Forming Units
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Thai, K. H., Thathireddy, A., Hsieh, M. H. Transurethral Induction of Mouse Urinary Tract Infection. J. Vis. Exp. (42), e2070, doi:10.3791/2070 (2010).
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