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反向转录-环介导的等温放大 (RT 灯) 测定尿液、血清和蚊子标本中的 Zika 病毒和管家基因
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Reverse Transcription-Loop-mediated Isothermal Amplification (RT-LAMP) Assay for Zika Virus and Housekeeping Genes in Urine, Serum, and Mosquito Samples
Please note that all translations are automatically generated.
Click here for the English version.
反向转录-环介导的等温放大 (RT 灯) 测定尿液、血清和蚊子标本中的 Zika 病毒和管家基因
DOI:
10.3791/58436-v
•
08:03 min
•
September 14, 2018
•
Sarah N. Bartolone
,
Maya O. Tree
,
Michael J. Conway
,
Michael B. Chancellor
3
,
Laura E. Lamb
3
1
Department of Urology
,
Beaumont Health System
,
2
Foundational Sciences, College of Medicine
,
Central Michigan University
,
3
Oakland University William Beaumont School of Medicine
Chapters
00:04
Title
01:01
RT-LAMP Primer Preparation
01:46
Sample Preparation
02:39
RT-LAMP Assay
03:49
RT-LAMP Analysis
05:15
Results: Analysis of RT-LAMP Reactions
06:44
Conclusion
Summary
Automatic Translation
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Automatic Translation
该协议提供了一种高效、低成本的方法, 用于检测人体尿液、血清样品或蚊子中的 Zika 病毒或控制靶, 采用反向转录环介导的等温放大 (RT 灯)。此方法不需要 RNA 隔离, 可以在30分钟内完成。
Tags
RT-LAMP
Zika Virus
Urine
Serum
Mosquito
Diagnosis
Infectious Disease
Dengue
Chikungunya
West Nile Virus
Primers
Sample Preparation
Master Mix
Isothermal Amplification
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