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Adapting 3′ Rapid Amplification of CDNA Ends to Map Transcripts in Cancer
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Genetics
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JoVE Journal Genetics
Adapting 3′ Rapid Amplification of CDNA Ends to Map Transcripts in Cancer

Adapting 3′ Rapid Amplification of CDNA Ends to Map Transcripts in Cancer

English

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12,077 Views

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09:38 min

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March 28, 2018

DOI:

10.3791/57318-v

DOI:
10.3791/57318-v

•

09:38 min
March 28, 2018

•

2 Views
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1Department of Pharmaceutical Sciences, College of Pharmacy and Health Sciences,Butler University

Summary

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The two different 3' rapid amplification of cDNA ends (3' RACE) protocols described here make use of two different DNA polymerases to map sequences that include a segment of the open reading frame (ORF), the stop codon, and the entire 3' UTR of a transcript using RNA obtained from different cancer cell lines.

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Cite this Article

Masamha, C. P., Todd, Z. Adapting 3' Rapid Amplification of CDNA Ends to Map Transcripts in Cancer. J. Vis. Exp. (133), e57318, doi:10.3791/57318 (2018).

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