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Reverse Transkription-Loop-vermittelten Isothermen Verstärkung (RT-Lampe) Assay für Zika-Virus und Housekeeping-Gene in Urin, Serum und Moskito-Proben
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Genetics
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JoVE Journal Genetics
Reverse Transcription-Loop-mediated Isothermal Amplification (RT-LAMP) Assay for Zika Virus and Housekeeping Genes in Urine, Serum, and Mosquito Samples
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Reverse Transkription-Loop-vermittelten Isothermen Verstärkung (RT-Lampe) Assay für Zika-Virus und Housekeeping-Gene in Urin, Serum und Moskito-Proben

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13,622 Views

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08:03 min

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September 14, 2018

DOI:

10.3791/58436-v

DOI:
10.3791/58436-v

•

08:03 min
September 14, 2018

•

2 Views
, , , ,
1Department of Urology,Beaumont Health System, 2Foundational Sciences, College of Medicine,Central Michigan University, 3Oakland University William Beaumont School of Medicine

Summary

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Dieses Protokoll bietet eine effiziente und kostengünstige Methode zum Zika Virus erkennen oder steuern Ziele im menschlichen Urin und Serum-Proben oder Mücken durch reverse Transkription Schleife-vermittelten Isothermen Amplifikation (RT-Lampe). Diese Methode erfordert keine RNA isoliert und kann innerhalb von 30 Minuten erfolgen.

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Cite this Article

Bartolone, S. N., Tree, M. O., Conway, M. J., Chancellor, M. B., Lamb, L. E. Reverse Transcription-Loop-mediated Isothermal Amplification (RT-LAMP) Assay for Zika Virus and Housekeeping Genes in Urine, Serum, and Mosquito Samples. J. Vis. Exp. (139), e58436, doi:10.3791/58436 (2018).

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