Journal
/
Genética
/
Reverse Transcription-Loop-mediated Isothermal Amplification (RT-LAMP) Assay for Zika Virus and Housekeeping Genes in Urine, Serum, and Mosquito Samples
JoVE Revista
Genética
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Genética
Reverse Transcription-Loop-mediated Isothermal Amplification (RT-LAMP) Assay for Zika Virus and Housekeeping Genes in Urine, Serum, and Mosquito Samples

Reverse Transcription-Loop-mediated Isothermal Amplification (RT-LAMP) Assay for Zika Virus and Housekeeping Genes in Urine, Serum, and Mosquito Samples

DOI:
10.3791/58436-v

•

08:03 min

•

September 14, 2018

•

, , , ,
1Department of Urology,Beaumont Health System, 2Foundational Sciences, College of Medicine,Central Michigan University, 3Oakland University William Beaumont School of Medicine

Capítulos

  • 00:04Título
  • 01:01RT-LAMP Primer Preparation
  • 01:46Sample Preparation
  • 02:39RT-LAMP Assay
  • 03:49RT-LAMP Analysis
  • 05:15Results: Analysis of RT-LAMP Reactions
  • 06:44Conclusion

Summary

Traducción Automática

Traducción Automática

This protocol provides an efficient and low-cost method to detect Zika virus or control targets in human urine and serum samples or in mosquitoes by reverse transcription loop-mediated isothermal amplification (RT-LAMP). This method does not require RNA isolation and can be done within 30 min.

Tags

RT-LAMPZika VirusUrineSerumMosquitoDiagnosisInfectious DiseaseDengueChikungunyaWest Nile VirusPrimersSample PreparationMaster MixIsothermal Amplification

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

The Use of Induced Somatic Sector Analysis (ISSA) for Studying Genes and Promoters Involved in Wood Formation and Secondary Stem Development

Measurement of Differentially Methylated INS DNA Species in Human Serum Samples as a Biomarker of Islet β Cell Death

Peptide-derived Method to Transport Genes and Proteins Across Cellular and Organellar Barriers in Plants

Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach

Chromatin Immunoprecipitation (ChIP) Protocol for Low-abundance Embryonic Samples

High Resolution Fluorescent In Situ Hybridization in Drosophila Embryos and Tissues Using Tyramide Signal Amplification

Transient Expression of Foreign Genes in Insect Cells (sf9) for Protein Functional Assay

Adapting 3′ Rapid Amplification of CDNA Ends to Map Transcripts in Cancer

Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing

Evaluation of a Universal Nested Reverse Transcription Polymerase Chain Reaction for the Detection of Lyssaviruses

Read Article