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Reverse transcriptie-lus-gemedieerde isotherme amplificatie (RT-LAMP) Assay voor Zika-Virus en huishouden genen in Urine, Serum en Mosquito monsters
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Genetics
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JoVE Journal Genetics
Reverse Transcription-Loop-mediated Isothermal Amplification (RT-LAMP) Assay for Zika Virus and Housekeeping Genes in Urine, Serum, and Mosquito Samples
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Reverse transcriptie-lus-gemedieerde isotherme amplificatie (RT-LAMP) Assay voor Zika-Virus en huishouden genen in Urine, Serum en Mosquito monsters

Nederlands

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13,622 Views

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08:03 min

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September 14, 2018

DOI:

10.3791/58436-v

DOI:
10.3791/58436-v

•

08:03 min
September 14, 2018

•

2 Views
, , , ,
1Department of Urology,Beaumont Health System, 2Foundational Sciences, College of Medicine,Central Michigan University, 3Oakland University William Beaumont School of Medicine

Summary

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Dit protocol biedt een efficiënte en goedkope methode speurder Zika virus te bepalen van doelen in menselijke urine en serum monsters of muggen door omgekeerde transcriptie lus-gemedieerde isotherme amplificatie (RT-LAMP). Deze methode vereist geen RNA isolatie en kan worden gedaan binnen 30 min.

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Cite this Article

Bartolone, S. N., Tree, M. O., Conway, M. J., Chancellor, M. B., Lamb, L. E. Reverse Transcription-Loop-mediated Isothermal Amplification (RT-LAMP) Assay for Zika Virus and Housekeeping Genes in Urine, Serum, and Mosquito Samples. J. Vis. Exp. (139), e58436, doi:10.3791/58436 (2018).

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