Journal
/
Genetics
/
Reverse transcriptie-lus-gemedieerde isotherme amplificatie (RT-LAMP) Assay voor Zika-Virus en huishouden genen in Urine, Serum en Mosquito monsters
JoVE Journal
Genetics
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Genetics
Reverse Transcription-Loop-mediated Isothermal Amplification (RT-LAMP) Assay for Zika Virus and Housekeeping Genes in Urine, Serum, and Mosquito Samples
Please note that all translations are automatically generated. Click here for the English version.

Reverse transcriptie-lus-gemedieerde isotherme amplificatie (RT-LAMP) Assay voor Zika-Virus en huishouden genen in Urine, Serum en Mosquito monsters

Nederlands

Automatically Generated

13,616 Views

•

08:03 min

•

September 14, 2018

DOI:

10.3791/58436-v

DOI:
10.3791/58436-v

•

08:03 min
September 14, 2018

•

2 Views
, , , ,
1Department of Urology,Beaumont Health System, 2Foundational Sciences, College of Medicine,Central Michigan University, 3Oakland University William Beaumont School of Medicine

Summary

Automatically generated

Dit protocol biedt een efficiënte en goedkope methode speurder Zika virus te bepalen van doelen in menselijke urine en serum monsters of muggen door omgekeerde transcriptie lus-gemedieerde isotherme amplificatie (RT-LAMP). Deze methode vereist geen RNA isolatie en kan worden gedaan binnen 30 min.

Explore More Videos

RT-LAMPZika VirusUrineSerumMosquitoDiagnosisInfectious DiseaseDengueChikungunyaWest Nile VirusPrimersSample PreparationMaster MixIsothermal Amplification

Read Article

Cite this Article

Bartolone, S. N., Tree, M. O., Conway, M. J., Chancellor, M. B., Lamb, L. E. Reverse Transcription-Loop-mediated Isothermal Amplification (RT-LAMP) Assay for Zika Virus and Housekeeping Genes in Urine, Serum, and Mosquito Samples. J. Vis. Exp. (139), e58436, doi:10.3791/58436 (2018).

Download .ris file

Copy

Share Video

.

Copy