Journal
/
Genetics
/
Evaluation of a Universal Nested Reverse Transcription Polymerase Chain Reaction for the Detection of Lyssaviruses
JoVE Journal
Genetics
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Genetics
Evaluation of a Universal Nested Reverse Transcription Polymerase Chain Reaction for the Detection of Lyssaviruses

Evaluation of a Universal Nested Reverse Transcription Polymerase Chain Reaction for the Detection of Lyssaviruses

English

Automatically Generated

8,246 Views

•

08:10 min

•

May 02, 2019

DOI:

10.3791/59428-v

DOI:
10.3791/59428-v

•

08:10 min
May 02, 2019

•

4 Views
, , , , , , ,
1Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary Medicine,Academy of Military Medical Sciences, 2Jilin Agricultural University, 3Jiangsu Co-innovation Centre for Prevention and Control of Important Animal Infectious Diseases and Zoonoses

Summary

Automatically generated

A pan-lyssavirus nested reverse transcription polymerase chain reaction has been developed to detect specifically all known lyssaviruses. Validation using rabies brain samples of different animal species showed that this method has a sensitivity and specificity equivalent to the gold standard fluorescent antibody test and could be used for routine rabies diagnosis.

Explore More Videos

LyssavirusNested RT-PCRUniversal DetectionAnimal Brain SpecimensBiological Fluid SpecimensRNA ExtractionReverse TranscriptionPCR ContaminationPositive ControlNegative ControlThermocycler

Read Article

Cite this Article

Wang, Y., Xu, W., Guo, H., Gong, W., He, B., Tu, Z., Tu, C., Feng, Y. Evaluation of a Universal Nested Reverse Transcription Polymerase Chain Reaction for the Detection of Lyssaviruses. J. Vis. Exp. (147), e59428, doi:10.3791/59428 (2019).

Download .ris file

Copy

Share Video

.

Copy