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An In Vitro Assay to Detect tRNA-Isopentenyl Transferase Activity
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Biochemistry
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JoVE Journal Biochemistry
An In Vitro Assay to Detect tRNA-Isopentenyl Transferase Activity

An In Vitro Assay to Detect tRNA-Isopentenyl Transferase Activity

English

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6,798 Views

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07:46 min

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October 08, 2018

DOI:

10.3791/58100-v

DOI:
10.3791/58100-v

•

07:46 min
October 08, 2018

•

2 Views
, , , , ,
1Department of Biology,Ball State University, 2Department of Genome Sciences,University of Washington, 3Department of Molecular, Cellular, and Developmental Biology,University of Michigan

Summary

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Here, we describe a protocol for the biochemical characterization of the yeast RNA-modifying enzyme, Mod5, and discuss how this protocol could be applied to other RNA-modifying enzymes.

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Chambers, A. E., Richardson, A. E., Read, D. F., Waller, T. J., Bernstein, D. A., Smaldino, P. J. An In Vitro Assay to Detect tRNA-Isopentenyl Transferase Activity. J. Vis. Exp. (140), e58100, doi:10.3791/58100 (2018).

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