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07:46 min
October 08, 2018
DOI:
10.3791/58100-v
在这里, 我们描述了一个协议的生化特性的酵母 rna 修饰酶, Mod5, 并讨论如何将该协议适用于其他 RNA 修饰酶。
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Chambers, A. E., Richardson, A. E., Read, D. F., Waller, T. J., Bernstein, D. A., Smaldino, P. J. An In Vitro Assay to Detect tRNA-Isopentenyl Transferase Activity. J. Vis. Exp. (140), e58100, doi:10.3791/58100 (2018).
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