Journal
/
生物化学
/
An In Vitro Assay to Detect tRNA-Isopentenyl Transferase Activity
JoVE 杂志
生物化学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 生物化学
An In Vitro Assay to Detect tRNA-Isopentenyl Transferase Activity

An In Vitro Assay to Detect tRNA-Isopentenyl Transferase Activity

6,786 Views

•

07:46 min

•

October 08, 2018

DOI:

10.3791/58100-v

DOI:
10.3791/58100-v

•

07:46 min
October 08, 2018

•

1 Views
, , , , ,
1Department of Biology,Ball State University, 2Department of Genome Sciences,University of Washington, 3Department of Molecular, Cellular, and Developmental Biology,University of Michigan

Tags

TRNA-Isopentenyl TransferaseIn Vitro AssayRNA ModificationBiochemical CharacterizationRecombinant EnzymesRNA Modifying EnzymesAcrylamide GelUrea GelTBE BufferRNA Isopentenylation AssayDMAPPMod52-mercaptoethanolRNA Precipitation

Read Article

Cite this Article

Chambers, A. E., Richardson, A. E., Read, D. F., Waller, T. J., Bernstein, D. A., Smaldino, P. J. An In Vitro Assay to Detect tRNA-Isopentenyl Transferase Activity. J. Vis. Exp. (140), e58100, doi:10.3791/58100 (2018).

Download .ris file

Copy

Share Video

.

Copy