Journal
/
생화학
/
An In Vitro Assay to Detect tRNA-Isopentenyl Transferase Activity
JoVE 신문
생화학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 생화학
An In Vitro Assay to Detect tRNA-Isopentenyl Transferase Activity

An In Vitro Assay to Detect tRNA-Isopentenyl Transferase Activity

6,798 Views

•

07:46 min

•

October 08, 2018

DOI:

10.3791/58100-v

DOI:
10.3791/58100-v

•

07:46 min
October 08, 2018

•

2 Views
, , , , ,
1Department of Biology,Ball State University, 2Department of Genome Sciences,University of Washington, 3Department of Molecular, Cellular, and Developmental Biology,University of Michigan

Summary

Automatically generated

Here, we describe a protocol for the biochemical characterization of the yeast RNA-modifying enzyme, Mod5, and discuss how this protocol could be applied to other RNA-modifying enzymes.

Explore More Videos

TRNA-Isopentenyl TransferaseIn Vitro AssayRNA ModificationBiochemical CharacterizationRecombinant EnzymesRNA Modifying EnzymesAcrylamide GelUrea GelTBE BufferRNA Isopentenylation AssayDMAPPMod52-mercaptoethanolRNA Precipitation

Read Article

Cite this Article

Chambers, A. E., Richardson, A. E., Read, D. F., Waller, T. J., Bernstein, D. A., Smaldino, P. J. An In Vitro Assay to Detect tRNA-Isopentenyl Transferase Activity. J. Vis. Exp. (140), e58100, doi:10.3791/58100 (2018).

Download .ris file

Copy

Share Video

.

Copy