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Quantification of Hypopigmentation Activity In Vitro
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Biochemistry
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JoVE Journal Biochemistry
Quantification of Hypopigmentation Activity In Vitro

Quantification of Hypopigmentation Activity In Vitro

English

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10,851 Views

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06:08 min

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March 06, 2019

DOI:

10.3791/58185-v

DOI:
10.3791/58185-v

•

06:08 min
March 06, 2019

•

10 Views
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1Department of Biotechnology, Graduate School of Life Sciences & Biotechnology, College of Life Sciences and Biotechnology,Korea University, 2JinWoo Bio Co. Ltd

Summary

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We describe three experimental methods for evaluating the hypopigmentation activity of chemicals in vitro: quantification of 1) cellular tyrosinase activity and 2) melanin content, and 3) measurement of melanin by cellular melanin staining and image analysis.

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Cite this Article

Kim, Y., Kim, M., Kweon, D., Lim, S., Lee, S. Quantification of Hypopigmentation Activity In Vitro. J. Vis. Exp. (145), e58185, doi:10.3791/58185 (2019).

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