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Um ensaio In Vitro para detectar a atividade de Transferase de tRNA-isopentenilo
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Biochemistry
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JoVE Journal Biochemistry
An In Vitro Assay to Detect tRNA-Isopentenyl Transferase Activity

Um ensaio In Vitro para detectar a atividade de Transferase de tRNA-isopentenilo

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6,798 Views

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07:46 min

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October 08, 2018

DOI:

10.3791/58100-v

DOI:
10.3791/58100-v

•

07:46 min
October 08, 2018

•

2 Views
, , , , ,
1Department of Biology,Ball State University, 2Department of Genome Sciences,University of Washington, 3Department of Molecular, Cellular, and Developmental Biology,University of Michigan

Summary

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Aqui, descrevemos um protocolo para a caracterização bioquímica de enzimas de RNA-modificando o fermento, Mod5 e discutir como este protocolo poderia ser aplicado a outras enzimas RNA-modificando.

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Cite this Article

Chambers, A. E., Richardson, A. E., Read, D. F., Waller, T. J., Bernstein, D. A., Smaldino, P. J. An In Vitro Assay to Detect tRNA-Isopentenyl Transferase Activity. J. Vis. Exp. (140), e58100, doi:10.3791/58100 (2018).

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