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Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors
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Chapters
- 00:05Introduction
- 00:24Prepare Assay Solutions for the Screen
- 01:48Prepare Enzyme Solutions for the Screen
- 02:52Run the Methylation Assay and Analyze the Data
- 04:29Results: Determination of Initial Velocities of DNA Methylation in the Presence and Absence of Potential Inhibitors
- 05:40Conclusion
DNA methyltransferases are potential cancer drug targets. Here, a protocol is presented to assess small molecules for DNA methyltransferase inhibition. This assay utilizes an endonuclease to couple DNA methylation to fluorescence generation and allows for enzyme activity to be monitored in real time.
