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Visualización de la Microbiota en agallas de garrapata por Whole-mount In Situ hibridación
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Genetics
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JoVE Journal Genetics
Visualization of Microbiota in Tick Guts by Whole-mount In Situ Hybridization
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Visualización de la Microbiota en agallas de garrapata por Whole-mount In Situ hibridación

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9,382 Views

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08:30 min

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June 01, 2018

DOI:

10.3791/57758-v

DOI:
10.3791/57758-v

•

08:30 min
June 01, 2018

•

16 Views
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1Department of Microbial Pathogenesis,Yale University School of Medicine, 2Program in Vertebrate Developmental Biology, Departments of Pediatrics and Genetics,Yale University School of Medicine, 3Department of Internal Medicine,Yale University School of Medicine

Summary

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Aquí, presentamos un protocolo evaluar espacial y temporal la presencia de microbiota viable en agallas de señal usando un acercamiento modificado todo montaje de hibridación in situ.

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Cite this Article

Moss, C. E., Robson, A., Fikrig, E., Narasimhan, S. Visualization of Microbiota in Tick Guts by Whole-mount In Situ Hybridization. J. Vis. Exp. (136), e57758, doi:10.3791/57758 (2018).

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