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Visualization of Microbiota in Tick Guts by Whole-mount In Situ Hybridization
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Genetik
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JoVE Journal Genetik
Visualization of Microbiota in Tick Guts by Whole-mount In Situ Hybridization

Visualization of Microbiota in Tick Guts by Whole-mount In Situ Hybridization

9,382 Views

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08:30 min

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June 01, 2018

DOI:

10.3791/57758-v

DOI:
10.3791/57758-v

•

08:30 min
June 01, 2018

•

16 Views
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1Department of Microbial Pathogenesis,Yale University School of Medicine, 2Program in Vertebrate Developmental Biology, Departments of Pediatrics and Genetics,Yale University School of Medicine, 3İç Hastalıkları Anabilim Dalı,Yale University School of Medicine

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Here, we present a protocol to spatially and temporally assess the presence of viable microbiota in tick guts using a modified whole-mount in situ hybridization approach.

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Cite this Article

Moss, C. E., Robson, A., Fikrig, E., Narasimhan, S. Visualization of Microbiota in Tick Guts by Whole-mount In Situ Hybridization. J. Vis. Exp. (136), e57758, doi:10.3791/57758 (2018).

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