Journal
/
Genética
/
Visualization of Microbiota in Tick Guts by Whole-mount In Situ Hybridization
JoVE Journal
Genética
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Genética
Visualization of Microbiota in Tick Guts by Whole-mount In Situ Hybridization

Visualization of Microbiota in Tick Guts by Whole-mount In Situ Hybridization

9,380 Views

•

08:30 min

•

June 01, 2018

DOI:

10.3791/57758-v

DOI:
10.3791/57758-v

•

08:30 min
June 01, 2018

•

16 Views
, , ,
1Department of Microbial Pathogenesis,Yale University School of Medicine, 2Program in Vertebrate Developmental Biology, Departments of Pediatrics and Genetics,Yale University School of Medicine, 3Department of Internal Medicine,Yale University School of Medicine

Summary

Automatically generated

Here, we present a protocol to spatially and temporally assess the presence of viable microbiota in tick guts using a modified whole-mount in situ hybridization approach.

Explore More Videos

MicrobiotaTick GutsWhole-mount In Situ HybridizationTick EntomologyBacteriaGene TranscriptsMEMFA SolutionRazor BladesSalivary GlandsNylon Mesh24-well Plate

Read Article

Cite this Article

Moss, C. E., Robson, A., Fikrig, E., Narasimhan, S. Visualization of Microbiota in Tick Guts by Whole-mount In Situ Hybridization. J. Vis. Exp. (136), e57758, doi:10.3791/57758 (2018).

Download .ris file

Copy

Share Video

.

Copy