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Visualization of Microbiota in Tick Guts by Whole-mount In Situ Hybridization
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JoVE Journal Genetik
Visualization of Microbiota in Tick Guts by Whole-mount In Situ Hybridization

Visualization of Microbiota in Tick Guts by Whole-mount In Situ Hybridization

DOI:
10.3791/57758-v

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08:30 min

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June 01, 2018

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, , ,
1Department of Microbial Pathogenesis,Yale University School of Medicine, 2Program in Vertebrate Developmental Biology, Departments of Pediatrics and Genetics,Yale University School of Medicine, 3Department of Internal Medicine,Yale University School of Medicine

Kapitel

  • 00:05Titel
  • 00:45Tick Gut Collection and Fixation
  • 02:06Construction of Mesh Sample Baskets and 24-well Basket Holder
  • 03:37In Situ Hybridization
  • 06:25Results: Tips for Success
  • 08:05Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Here, we present a protocol to spatially and temporally assess the presence of viable microbiota in tick guts using a modified whole-mount in situ hybridization approach.

Tags

MicrobiotaTick GutsWhole-mount In Situ HybridizationTick EntomologyBacteriaGene TranscriptsMEMFA SolutionRazor BladesSalivary GlandsNylon Mesh24-well Plate

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