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Visualization of Microbiota in Tick Guts by Whole-mount In Situ Hybridization
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JoVE Journal 遺伝学
Visualization of Microbiota in Tick Guts by Whole-mount In Situ Hybridization

Visualization of Microbiota in Tick Guts by Whole-mount In Situ Hybridization

DOI:
10.3791/57758-v

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08:30 min

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June 01, 2018

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, , ,
1Department of Microbial Pathogenesis,Yale University School of Medicine, 2Program in Vertebrate Developmental Biology, Departments of Pediatrics and Genetics,Yale University School of Medicine, 3内科学科,Yale University School of Medicine

章

  • 00:05標題
  • 00:45Tick Gut Collection and Fixation
  • 02:06Construction of Mesh Sample Baskets and 24-well Basket Holder
  • 03:37In Situ Hybridization
  • 06:25Results: Tips for Success
  • 08:05Conclusion

概要

自動翻訳

自動翻訳

Here, we present a protocol to spatially and temporally assess the presence of viable microbiota in tick guts using a modified whole-mount in situ hybridization approach.

タグ

MicrobiotaTick GutsWhole-mount In Situ HybridizationTick EntomologyBacteriaGene TranscriptsMEMFA SolutionRazor BladesSalivary GlandsNylon Mesh24-well Plate

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