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Visualization of Microbiota in Tick Guts by Whole-mount In Situ Hybridization
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Visualization of Microbiota in Tick Guts by Whole-mount In Situ Hybridization

Visualization of Microbiota in Tick Guts by Whole-mount In Situ Hybridization

9,380 Views

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08:30 min

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June 01, 2018

DOI:

10.3791/57758-v

DOI:
10.3791/57758-v

•

08:30 min
June 01, 2018

•

16 Views
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1Department of Microbial Pathogenesis,Yale University School of Medicine, 2Program in Vertebrate Developmental Biology, Departments of Pediatrics and Genetics,Yale University School of Medicine, 3Department of Internal Medicine,Yale University School of Medicine

Summary

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Here, we present a protocol to spatially and temporally assess the presence of viable microbiota in tick guts using a modified whole-mount in situ hybridization approach.

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Moss, C. E., Robson, A., Fikrig, E., Narasimhan, S. Visualization of Microbiota in Tick Guts by Whole-mount In Situ Hybridization. J. Vis. Exp. (136), e57758, doi:10.3791/57758 (2018).

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