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Visualisation du microbiote dans Guts Tick par hybridation entier-Montez In Situ
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Genetics
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JoVE Journal Genetics
Visualization of Microbiota in Tick Guts by Whole-mount In Situ Hybridization
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Visualisation du microbiote dans Guts Tick par hybridation entier-Montez In Situ

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9,382 Views

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08:30 min

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June 01, 2018

DOI:

10.3791/57758-v

DOI:
10.3791/57758-v

•

08:30 min
June 01, 2018

•

16 Views
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1Department of Microbial Pathogenesis,Yale University School of Medicine, 2Program in Vertebrate Developmental Biology, Departments of Pediatrics and Genetics,Yale University School of Medicine, 3Department of Internal Medicine,Yale University School of Medicine

Summary

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Nous présentons ici un protocole pour spatialement et temporellement évaluer la présence de la microflore viable dans les tripes de tique à l’aide d’une approche modifiée entier-Montez l’hybridation in situ.

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Cite this Article

Moss, C. E., Robson, A., Fikrig, E., Narasimhan, S. Visualization of Microbiota in Tick Guts by Whole-mount In Situ Hybridization. J. Vis. Exp. (136), e57758, doi:10.3791/57758 (2018).

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