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Journal
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Neuroscience
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鼠脑切片偶联微电极阵列癫痫活性的记录与调控
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal
Neuroscience
Recording and Modulation of Epileptiform Activity in Rodent Brain Slices Coupled to Microelectrode Arrays
Please note that all translations are automatically generated.
Click here for the English version.
鼠脑切片偶联微电极阵列癫痫活性的记录与调控
DOI:
10.3791/57548-v
•
10:24 min
•
May 15, 2018
•
Gabriella Panuccio
,
Ilaria Colombi
,
Michela Chiappalone
2
1
Department of Neuroscience and Brain Technologies
,
Istituto Italiano di Tecnologia
,
2
Rehab Technologies
,
Istituto Italiano di Tecnologia
Chapters
00:04
Title
00:39
Preparation of the MEA/Custom Chamber Assembly
01:15
Preparation of the Recording Bench
02:52
Preparation of the MEA Set-up
04:39
MEA Live Mapping
06:33
Recording and Electrical Modulation of the Epileptiform Activity
08:49
Results: Representative Experiment of Electrical Modulation of Ictogenesis Using Brain Slices Coupled to MEAs
09:21
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们说明如何使用微电极阵列对 4-氨基吡啶诱导的啮齿动物脑切片中的癫痫活性进行记录和电调制。一个自定义记录室保持组织的可行性在长期的实验课。实时电极映射和激发对的选择由自定义图形用户界面执行。
Tags
Epileptiform Activity
Rodent Brain Slices
Microelectrode Arrays
Neuromodulation
Epilepsy Treatment
Brain Circuits
MEA
ACSF
4AP
Hippocampus-cortex
Brain Slice Preparation
Perfusion
Recording Chamber
Heating
Amplifier
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