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Evaluation of Synapse Density in Hippocampal Rodent Brain Slices
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Evaluation of Synapse Density in Hippocampal Rodent Brain Slices

Evaluation of Synapse Density in Hippocampal Rodent Brain Slices

DOI:
10.3791/56153-v

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07:44 min

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October 06, 2017

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, , , ,
1Department of Cell and Developmental Biology,University College London

Chapters

  • 00:05Title
  • 00:41Hippocampal Slice Preparation
  • 02:17Immunofluorescence for Synaptic Markers
  • 04:34Imaging Using Confocal Laser Scanning Microscopy
  • 06:04Synapse Density in Hippocampal Slices from iDkk1 Mice
  • 07:17Conclusion

Summary

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Automatic Translation

A protocol for accurately identifying and analyzing synapses in hippocampal slices using immunofluorescence is outlined in this article.

Tags

ImmunostainingSynapse DensityHippocampusRodent Brain SlicesNeurodegenerative DiseasesVibratomeACSFParaformaldehydeImmunofluorescent StainingVGlut1 AntibodySecondary Antibodies

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