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Recording Synaptic Plasticity in Acute Hippocampal Slices Maintained in a Small-volume Recycling-, Perfusion-, and Submersion-type Chamber System
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Neuroscience
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JoVE Journal Neuroscience
Recording Synaptic Plasticity in Acute Hippocampal Slices Maintained in a Small-volume Recycling-, Perfusion-, and Submersion-type Chamber System

Recording Synaptic Plasticity in Acute Hippocampal Slices Maintained in a Small-volume Recycling-, Perfusion-, and Submersion-type Chamber System

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11,272 Views

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09:51 min

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January 01, 2018

DOI:

10.3791/55936-v

DOI:
10.3791/55936-v

•

09:51 min
January 01, 2018

•

3 Views
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1The Institutes of Brain Science, the State Key Laboratory of Medical Neurobiology, the Collaborative Innovation Center for Brain Science,Fudan University

Summary

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This protocol describes the stabilization of the oxygen level in a small volume of recycled buffer and methodological aspects of recording activity-dependent synaptic plasticity in submerged acute hippocampal slices.

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Synaptic PlasticityAcute Hippocampal SlicesRecycling-perfusion-submersion ChamberCarbogenationField Potential RecordingsNeuroplasticitySynaptic TransmissionMemory FormationOxygen StabilizationAcute Slice PreparationVibratomeHippocampal FormationRecycling SystemCarbogen Supply

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Weng, W., Li, D., Peng, C., Behnisch, T. Recording Synaptic Plasticity in Acute Hippocampal Slices Maintained in a Small-volume Recycling-, Perfusion-, and Submersion-type Chamber System. J. Vis. Exp. (131), e55936, doi:10.3791/55936 (2018).

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