Journal
/
神经科学
/
Recording and Modulation of Epileptiform Activity in Rodent Brain Slices Coupled to Microelectrode Arrays
JoVE 杂志
神经科学
This content is Free Access.
JoVE 杂志 神经科学
Recording and Modulation of Epileptiform Activity in Rodent Brain Slices Coupled to Microelectrode Arrays

Recording and Modulation of Epileptiform Activity in Rodent Brain Slices Coupled to Microelectrode Arrays

14,177 Views

•

10:24 min

•

May 15, 2018

DOI:

10.3791/57548-v

DOI:
10.3791/57548-v

•

10:24 min
May 15, 2018

•

21 Views
, ,
1Department of Neuroscience and Brain Technologies,Istituto Italiano di Tecnologia, 2Rehab Technologies,Istituto Italiano di Tecnologia

Summary

Automatically generated

We illustrate how to perform recording and electrical modulation of 4-aminopyridine-induced epileptiform activity in rodent brain slices using microelectrode arrays. A custom recording chamber maintains tissue viability throughout prolonged experimental sessions. Live electrode mapping and selection of stimulating pairs are performed by a custom graphical user interface.

Explore More Videos

Epileptiform ActivityRodent Brain SlicesMicroelectrode ArraysNeuromodulationEpilepsy TreatmentBrain CircuitsMEAACSF4APHippocampus-cortexBrain Slice PreparationPerfusionRecording ChamberHeatingAmplifier

Read Article

Cite this Article

Panuccio, G., Colombi, I., Chiappalone, M. Recording and Modulation of Epileptiform Activity in Rodent Brain Slices Coupled to Microelectrode Arrays. J. Vis. Exp. (135), e57548, doi:10.3791/57548 (2018).

Download .ris file

Copy

Share Video

.

Copy