Journal
/
Neuroscience
/
Quantifying Subcellular Ubiquitin-proteasome Activity in the Rodent Brain
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Quantifying Subcellular Ubiquitin-proteasome Activity in the Rodent Brain

Quantifying Subcellular Ubiquitin-proteasome Activity in the Rodent Brain

English

Automatically Generated

6,519 Views

•

09:25 min

•

May 21, 2019

DOI:

10.3791/59695-v

DOI:
10.3791/59695-v

•

09:25 min
May 21, 2019

•

26 Views
, ,
1Department of Animal and Poultry Sciences,Virginia Polytechnic Institute and State University, 2School of Neuroscience,Virginia Polytechnic Institute and State University

Summary

Automatically generated

This protocol is designed to efficiently quantify ubiquitin-proteasome system (UPS) activity in different cellular compartments of the rodent brain. Users are able to examine UPS functioning in nuclear, cytoplasmic and synaptic fractions in the same animal, reducing the amount of time and number of animals needed to perform these complex analyses.

Explore More Videos

Subcellular FractionationUbiquitin-proteasome SystemProtein UbiquitinationProteasome ActivityRodent BrainCytoplasmic FractionNuclear FractionSynaptic FractionCentrifugation

Read Article

Cite this Article

McFadden, T., Devulapalli, R. K., Jarome, T. J. Quantifying Subcellular Ubiquitin-proteasome Activity in the Rodent Brain. J. Vis. Exp. (147), e59695, doi:10.3791/59695 (2019).

Download .ris file

Copy

Share Video

.

Copy