Journal
/
Immunology and Infection
/
基于流式细胞仪的 Zika 病毒疗养血清测定登革热病毒的体外抗体依赖性
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
A Simple Flow Cytometry Based Assay to Determine In Vitro Antibody Dependent Enhancement of Dengue Virus Using Zika Virus Convalescent Serum
Please note that all translations are automatically generated. Click here for the English version.

基于流式细胞仪的 Zika 病毒疗养血清测定登革热病毒的体外抗体依赖性

DOI:
10.3791/57371-v

•

07:06 min

•

April 10, 2018

•

,
1Department of Microbiology and Immunology,Uniformed Services University

Chapters

  • 00:04Title
  • 00:47Day One: Infection of K562 Cells with DENV in the Presence of ZIKV Convalescent
  • 03:53Day 3: Fixation and Flow Cytometry
  • 04:51Data Analysis
  • 05:31Results: Antibody-dependent Enhancement of Dengue Virus
  • 06:31Conclusion

Summary

Automatic Translation

Automatic Translation

我们描述了一个简单和简单的协议, 以测量抗体依赖性增强感染的 Zika 病毒恢复血清使用登革热病毒报告病毒粒子。

Tags

Flow CytometryAntibody Dependent EnhancementDengue VirusZika VirusConvalescent SerumRhesus MacaquesIn VitroVirus InfectionRPMISerial DilutionsDengue Virus SerotypesK562 CellsTrypan BlueHemocytometerCentrifugation

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

可视登革病毒通过的Alexa Fluor标签

基于图像的流式细胞仪技术来评估变化粒细胞功能<em>在体外</em

中性粒细胞分离和分析，以确定他们的淋巴瘤细胞的敏感性对治疗药物的作用

一种简单有效的方法构建突变牛痘病毒载体

兹卡病毒感染细胞培养系统和<em>体外</em>干扰素的预防作用

来自新世界Zika病毒感染性克隆的重组病毒的救援和表征

Zika 病毒特定诊断表位发现

小鼠与人肺泡巨噬细胞的分离和体外培养

快速、安全、简便的人工床边核酸提取法检测全血标本中的病毒

原代人细胞中寨卡病毒抗体依赖性增强的定量研究

Read Article