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Neutrophil Isolation and Analysis to Determine their Role in Lymphoma Cell Sensitivity to Therapeutic Agents
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Chapters
- 00:05Title
- 01:02Primary Leukemic Cell and Neutrophil Isolation
- 05:25Primary Leukemic Cell and Neutrophil Purity and Co-culture Phenotype Analysis by Flow Cytometry
- 07:583D Culture Setup and Phenotype Analysis
- 10:59Results: Analysis of the Role of Neutrophils in Lymphoma Cell Sensitivity to Therapeutic Agents
- 13:09Conclusion
Neutrophils are the most abundant type of white blood cells. The isolation of neutrophils from human blood by density gradient separation method and the differentiation of human promyelocytic (HL60) cells along the granulocytic pathway are described here; to test their role on sensitivity of lymphoma cells to anti-lymphoma agents.
