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A Simple Flow Cytometry Based Assay to Determine In Vitro Antibody Dependent Enhancement of Dengue Virus Using Zika Virus Convalescent Serum
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Immunology and Infection
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JoVE Journal Immunology and Infection
A Simple Flow Cytometry Based Assay to Determine In Vitro Antibody Dependent Enhancement of Dengue Virus Using Zika Virus Convalescent Serum

A Simple Flow Cytometry Based Assay to Determine In Vitro Antibody Dependent Enhancement of Dengue Virus Using Zika Virus Convalescent Serum

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8,608 Views

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07:06 min

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April 10, 2018

DOI:

10.3791/57371-v

DOI:
10.3791/57371-v

•

07:06 min
April 10, 2018

•

7 Views
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1Department of Microbiology and Immunology,Uniformed Services University

Summary

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We describe a simple and easy protocol for measuring antibody dependent enhancement of infection by Zika virus convalescent serum using Dengue virus Reporter Viral Particles.

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Cite this Article

Valiant, W. G., Mattapallil, J. J. A Simple Flow Cytometry Based Assay to Determine In Vitro Antibody Dependent Enhancement of Dengue Virus Using Zika Virus Convalescent Serum. J. Vis. Exp. (134), e57371, doi:10.3791/57371 (2018).

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