Journal
/
Immunology and Infection
/
Visualizing Dengue Virus through Alexa Fluor Labeling
JoVE Journal
Immunology and Infection
Author Produced
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Visualizing Dengue Virus through Alexa Fluor Labeling

Visualizing Dengue Virus through Alexa Fluor Labeling

DOI:
10.3791/3168-v

•

09:11 min

•

July 09, 2011

•

, ,
1Defence Medical and Environmental Research Institute,DSO National Laboratories, 2Program in Emerging Infectious Diseases,Duke-NUS Graduate Medical School, 3Program in Emerging Infectious Diseases,Duke-NUS Graduate Medical School

Chapters

  • 00:05Title
  • 01:20Alexa Fluor Labeling of Dengue Virus
  • 03:39Assessing Viability and Degree of Labeling

Summary

Automatic Translation

Automatic Translation

Taking advantage of the advancements in fluorophore development and imaging technology, a simple method of Alexa Fluor labeling of dengue virus was devised to visualize the early interactions between virus and cell.

Tags

VisualizingDengue VirusAlexa Fluor LabelingInteractionVirus And CellInfectionDisease PathogenesisVaccineTherapeutic DesignFluorophores DevelopmentImaging TechnologyDengue PathogenesisDengue InfectionsEnveloped Dengue VirusEnvelope GlycoproteinNucleocapsid ShellRNA GenomeAmine Reactive DyeImmunofluorescent MicroscopyLabeling MethodAlexa Fluor Succinimidyl Ester DyeSodium Bicarbonate BufferLive Virus Labeling

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Neutrophil Isolation and Analysis to Determine their Role in Lymphoma Cell Sensitivity to Therapeutic Agents

Evaluation of the Efficacy And Toxicity of RNAs Targeting HIV-1 Production for Use in Gene or Drug Therapy

Rescue and Characterization of Recombinant Virus from a New World Zika Virus Infectious Clone

Visualizing Macrophage Extracellular Traps Using Confocal Microscopy

A Simple Flow Cytometry Based Assay to Determine In Vitro Antibody Dependent Enhancement of Dengue Virus Using Zika Virus Convalescent Serum

Rapid, Safe, and Simple Manual Bedside Nucleic Acid Extraction for the Detection of Virus in Whole Blood Samples

Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction

Identification of Coding and Non-coding RNA Classes Expressed in Swine Whole Blood

A Murine Model of Dengue Virus-induced Acute Viral Encephalitis-like Disease

In Vitro ELISA Test to Evaluate Rabies Vaccine Potency

Read Article