Journal
/
Immunology and Infection
/
Quantification of Antibody-dependent Enhancement of the Zika Virus in Primary Human Cells
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Quantification of Antibody-dependent Enhancement of the Zika Virus in Primary Human Cells

Quantification of Antibody-dependent Enhancement of the Zika Virus in Primary Human Cells

English

Automatically Generated

8,605 Views

•

07:53 min

•

January 18, 2019

DOI:

10.3791/58691-v

DOI:
10.3791/58691-v

•

07:53 min
January 18, 2019

•

5 Views
, , , ,
1Department of Microbiology,Boston University School of Medicine, 2National Emerging Infectious Diseases Laborator,Boston University School of Medicine, 3Department of Entomology,Kansas State University

Summary

Automatically generated

We describe a method to evaluate the effect of pre-existing immunity against dengue virus on the Zika virus infection by using human serum, primary human cells, and infection quantification by quantitative real-time polymerase chain reaction.

Explore More Videos

Antibody-dependent EnhancementZika VirusPrimary Human CellsDengue VirusPre-existing ImmunitySerum SamplesVirus InfectionCell CultureImmune ComplexesCell LysisQuantification

Read Article

Cite this Article

Asad, S., Feitosa-Suntheimer, F., Gold, A., Londono-Renteria, B., Colpitts, T. M. Quantification of Antibody-dependent Enhancement of the Zika Virus in Primary Human Cells. J. Vis. Exp. (143), e58691, doi:10.3791/58691 (2019).

Download .ris file

Copy

Share Video

.

Copy