Journal
/
Immunology and Infection
/
Isolation and In Vitro Culture of Murine and Human Alveolar Macrophages
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Isolation and In Vitro Culture of Murine and Human Alveolar Macrophages

Isolation and In Vitro Culture of Murine and Human Alveolar Macrophages

DOI:
10.3791/57287-v

•

09:09 min

•

April 20, 2018

•

, , ,
1Norton Thoracic Institute,St. Joseph’s Hospital and Medical Center, 2Flow Cytometry Core,St. Joseph’s Hospital and Medical Center

Chapters

  • 00:04Title
  • 00:50Isolation of Alveolar Macrophages (AMs) from Murine Bronchoalveolar Lavage (BAL) Fluid
  • 03:58Isolation of AMs from Mouse Lung: Single-cell Suspension
  • 06:04In Vitro Culture of AMs
  • 07:06Results: Characteristics of Mouse Alveolar Macrophages
  • 07:42Conclusion

Summary

Automatic Translation

Automatic Translation

This communication describes methodologies for isolation and culture of alveolar macrophages from humans and murine models for experimental purposes.

Tags

Alveolar MacrophagesIn Vitro CultureMurineHumanLungBronchoalveolar LavagePulmonary ImmunologyRespiratory InfectionInflammationAutoimmunityCell IsolationTracheal CatheterizationPerfusion

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Human T Lymphocyte Isolation, Culture and Analysis of Migration In Vitro

Flow Cytometric Isolation of Primary Murine Type II Alveolar Epithelial Cells for Functional and Molecular Studies

Establishment of an In vitro System to Study Intracellular Behavior of Candida glabrata in Human THP-1 Macrophages

Isolation of Human Monocytes by Double Gradient Centrifugation and Their Differentiation to Macrophages in Teflon-coated Cell Culture Bags

Assessing Anti-fungal Activity of Isolated Alveolar Macrophages by Confocal Microscopy

Isolation of Murine Peritoneal Macrophages to Carry Out Gene Expression Analysis Upon Toll-like Receptors Stimulation

Culture of Macrophage Colony-stimulating Factor Differentiated Human Monocyte-derived Macrophages

Quantifying Human Monocyte Chemotaxis In Vitro and Murine Lymphocyte Trafficking In Vivo

Isolation of Salmonella typhimurium-containing Phagosomes from Macrophages

Isolation, Transfection, and Culture of Primary Human Monocytes

Read Article