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Üç boyutlu görüntü oluşturma ve çözümleme-in Immunolabeled, açıklık insan plasental Villous damar ağları
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Three-dimensional Rendering and Analysis of Immunolabeled, Clarified Human Placental Villous Vascular Networks
DOI:

09:33 min

March 29, 2018

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Chapters

  • 00:04Title
  • 01:17Villous Tree Dissection and Immunofluorescent Staining
  • 03:16Clarification of the Tissue
  • 04:07Mounting for Microscopy and Confocal Microscopy
  • 06:04Reversing the Clearing
  • 06:33Results: Use of Confocal Microscopy, Immunostaining, and Reverse Clearing to Analyze the Vascularization of Placental Network
  • 08:21Conclusion

Summary

Automatic Translation

Bu çalışmada tersinir doku takas, immunostaining, 3D işleme ve analiz insan plasenta villus örneklerinde sırasına 1-2 mm3damar ağları için bir protokol sunar.

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