Journal
/
발생학
/
Three-dimensional Rendering and Analysis of Immunolabeled, Clarified Human Placental Villous Vascular Networks
JoVE 신문
발생학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 발생학
Three-dimensional Rendering and Analysis of Immunolabeled, Clarified Human Placental Villous Vascular Networks

Three-dimensional Rendering and Analysis of Immunolabeled, Clarified Human Placental Villous Vascular Networks

9,536 Views

•

09:33 min

•

March 29, 2018

DOI:

10.3791/57099-v

DOI:
10.3791/57099-v

•

09:33 min
March 29, 2018

•

5 Views
, , , , , , , ,
1The Institute for Basic Research,The New York State Office for People with Developmental Disabilities, 2Placental Analytics LLC, 3Visikol Inc.

Summary

Automatically generated

This study presents a protocol for the reversible tissue clearing, immunostaining, 3D-rendering and analysis of vascular networks in human placenta villi samples on the order of 1 - 2 mm3.

Explore More Videos

3D RenderingImmunolabeledClarifiedPlacental Villous Vascular NetworksVascular AnalysisImmunolabelingCD31CK7Fluorescent LabelingMicroscopyPlacental Growth RestrictionPreterm Birth

Read Article

Cite this Article

Merz, G., Schwenk, V., Shah, R., Salafia, C., Necaise, P., Joyce, M., Villani, T., Johnson, M., Crider, N. Three-dimensional Rendering and Analysis of Immunolabeled, Clarified Human Placental Villous Vascular Networks. J. Vis. Exp. (133), e57099, doi:10.3791/57099 (2018).

Download .ris file

Copy

Share Video

.

Copy