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Developmental Biology
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三次元レンダリングとカルビンディン抗体、解析人間の胎盤絨毛血管のネットワークを明らかにしました。
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
Three-dimensional Rendering and Analysis of Immunolabeled, Clarified Human Placental Villous Vascular Networks
Please note that all translations are automatically generated.
Click here for the English version.
三次元レンダリングとカルビンディン抗体、解析人間の胎盤絨毛血管のネットワークを明らかにしました。
DOI:
10.3791/57099-v
•
09:33 min
•
March 29, 2018
•
George Merz
,
Valerie Schwenk
,
Ruchit Shah
,
Carolyn Salafia
2
,
Phillip Necaise
,
Michael Joyce
,
Tom Villani
,
Michael Johnson
,
Nick Crider
1
The Institute for Basic Research
,
The New York State Office for People with Developmental Disabilities
,
2
Placental Analytics LLC
,
3
Visikol Inc.
Chapters
00:04
Title
01:17
Villous Tree Dissection and Immunofluorescent Staining
03:16
Clarification of the Tissue
04:07
Mounting for Microscopy and Confocal Microscopy
06:04
Reversing the Clearing
06:33
Results: Use of Confocal Microscopy, Immunostaining, and Reverse Clearing to Analyze the Vascularization of Placental Network
08:21
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本研究は、リバーシブルの組織免疫染色、3 D レンダリングやひと胎盤絨毛サンプル 1-2 mm
3
程度の血管ネットワークの解析をクリアするためのプロトコルを示します。
Tags
3D Rendering
Immunolabeled
Clarified
Placental Villous Vascular Networks
Vascular Analysis
Immunolabeling
CD31
CK7
Fluorescent Labeling
Microscopy
Placental Growth Restriction
Preterm Birth
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