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Three-dimensional Inflammatory Human Tissue Equivalents of Gingiva
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Developmental Biology
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JoVE Journal Developmental Biology
Three-dimensional Inflammatory Human Tissue Equivalents of Gingiva

Three-dimensional Inflammatory Human Tissue Equivalents of Gingiva

DOI:
10.3791/57157-v

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08:43 min

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April 03, 2018

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, ,
1Department of Pharmacology,The Nippon Dental University, 2Department of Oral and Maxillofacial Surgery,The Nippon Dental University Hospital

Chapters

  • 00:04Title
  • 00:46Preparation of 3D Human Tissue Equivalents of Gingiva
  • 04:17Preparation of Wounded and Inflammatory GTE
  • 05:46Results: Characterize the 3D Inflammatory Human Tissue Equivalents of Gingiva (iGTE)
  • 07:30Conclusion

Summary

Automatic Translation

Automatic Translation

The goal of the protocol is to build an inflammatory human gingiva model in vitro. This tissue model co-cultivates three types of human cells, HaCaT keratinocytes, gingival fibroblasts, and THP-1 macrophages, under three-dimensional conditions. This model can be applied to investigating periodontal diseases, such as gingivitis and periodontitis.

Tags

3DInflammatoryGingivaIn VitroHuman CellsWound HealingInflammationPeriodontal DiseasesCollagenHGFHaCaTCulture InsertCulture MediumCell SuspensionCo-culture

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