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Immunolabeled, 澄清人胎盘长柔毛血管网络的三维渲染与分析
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Developmental Biology
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JoVE Journal Developmental Biology
Three-dimensional Rendering and Analysis of Immunolabeled, Clarified Human Placental Villous Vascular Networks
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Immunolabeled, 澄清人胎盘长柔毛血管网络的三维渲染与分析

DOI:
10.3791/57099-v

•

09:33 min

•

March 29, 2018

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, , , , , , , ,
1The Institute for Basic Research,The New York State Office for People with Developmental Disabilities, 2Placental Analytics LLC, 3Visikol Inc.

Chapters

  • 00:04Title
  • 01:17Villous Tree Dissection and Immunofluorescent Staining
  • 03:16Clarification of the Tissue
  • 04:07Mounting for Microscopy and Confocal Microscopy
  • 06:04Reversing the Clearing
  • 06:33Results: Use of Confocal Microscopy, Immunostaining, and Reverse Clearing to Analyze the Vascularization of Placental Network
  • 08:21Conclusion

Summary

Automatic Translation

Automatic Translation

本研究提出了一种可逆转组织清除、染色、3 d 渲染和分析人胎盘绒毛样品中血管网络的方法, 其顺序为 1-2 毫米3。

Tags

3D RenderingImmunolabeledClarifiedPlacental Villous Vascular NetworksVascular AnalysisImmunolabelingCD31CK7Fluorescent LabelingMicroscopyPlacental Growth RestrictionPreterm Birth

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