Journal
/
Developmental Biology
/
Three-dimensional Rendering and Analysis of Immunolabeled, Clarified Human Placental Villous Vascular Networks
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
Three-dimensional Rendering and Analysis of Immunolabeled, Clarified Human Placental Villous Vascular Networks

Three-dimensional Rendering and Analysis of Immunolabeled, Clarified Human Placental Villous Vascular Networks

English

Automatically Generated

9,540 Views

•

09:33 min

•

March 29, 2018

DOI:

10.3791/57099-v

DOI:
10.3791/57099-v

•

09:33 min
March 29, 2018

•

5 Views
, , , , , , , ,
1The Institute for Basic Research,The New York State Office for People with Developmental Disabilities, 2Placental Analytics LLC, 3Visikol Inc.

Summary

Automatically generated

This study presents a protocol for the reversible tissue clearing, immunostaining, 3D-rendering and analysis of vascular networks in human placenta villi samples on the order of 1 - 2 mm3.

Explore More Videos

3D RenderingImmunolabeledClarifiedPlacental Villous Vascular NetworksVascular AnalysisImmunolabelingCD31CK7Fluorescent LabelingMicroscopyPlacental Growth RestrictionPreterm Birth

Read Article

Cite this Article

Merz, G., Schwenk, V., Shah, R., Salafia, C., Necaise, P., Joyce, M., Villani, T., Johnson, M., Crider, N. Three-dimensional Rendering and Analysis of Immunolabeled, Clarified Human Placental Villous Vascular Networks. J. Vis. Exp. (133), e57099, doi:10.3791/57099 (2018).

Download .ris file

Copy

Share Video

.

Copy