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Dreidimensionale Darstellung und Analyse der Immunolabeled, geklärt menschlichen Plazenta Zotten vaskulärer Netzwerke
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Developmental Biology
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JoVE Journal Developmental Biology
Three-dimensional Rendering and Analysis of Immunolabeled, Clarified Human Placental Villous Vascular Networks
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Dreidimensionale Darstellung und Analyse der Immunolabeled, geklärt menschlichen Plazenta Zotten vaskulärer Netzwerke

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9,536 Views

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09:33 min

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March 29, 2018

DOI:

10.3791/57099-v

DOI:
10.3791/57099-v

•

09:33 min
March 29, 2018

•

5 Views
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1The Institute for Basic Research,The New York State Office for People with Developmental Disabilities, 2Placental Analytics LLC, 3Visikol Inc.

Summary

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Diese Studie stellt ein Protokoll für die reversible Gewebe, clearing, Immunostaining, 3D-Darstellung und Analyse von vaskulären Netzwerken im menschlichen Plazenta Zotten Proben in der Größenordnung von 1 bis 2 mm3.

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Cite this Article

Merz, G., Schwenk, V., Shah, R., Salafia, C., Necaise, P., Joyce, M., Villani, T., Johnson, M., Crider, N. Three-dimensional Rendering and Analysis of Immunolabeled, Clarified Human Placental Villous Vascular Networks. J. Vis. Exp. (133), e57099, doi:10.3791/57099 (2018).

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