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生体内でひと脂肪由来間葉系幹細胞が脂溶性膜の蛍光色素をラット膝関節変形性関節症モデルでの追跡
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
In Vivo Tracking of Human Adipose-derived Mesenchymal Stem Cells in a Rat Knee Osteoarthritis Model with Fluorescent Lipophilic Membrane Dye
DOI:

07:31 min

October 08, 2017

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Chapters

  • 00:05Title
  • 00:58Surgical Rat Knee Osteoarthritis (KOA) Induction
  • 02:36Human Mesenchymal Stem Cell Fluorescent Dye Labelling and In Vivo Fluorescent Imaging
  • 05:16Results: Representative Rat KOA Induction and haMSC Imaging Analyses
  • 06:49Conclusion

Summary

Automatic Translation

このプロトコルでは、効率的に遠赤色蛍光 (IA) の関節内注入によるラット膝変形性関節症 (KOA) モデルで、セルの永続性とひと脂肪由来間葉系幹細胞 (haMSCs) の体内を監視する方法について説明します。

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