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In Vivo Rilevamento di umani cellule staminali mesenchimali adiposo-derivate in un modello di osteoartrite del ginocchio di ratto con colorante fluorescente lipofilico membrana
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Developmental Biology
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JoVE Journal Developmental Biology
In Vivo Tracking of Human Adipose-derived Mesenchymal Stem Cells in a Rat Knee Osteoarthritis Model with Fluorescent Lipophilic Membrane Dye
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In Vivo Rilevamento di umani cellule staminali mesenchimali adiposo-derivate in un modello di osteoartrite del ginocchio di ratto con colorante fluorescente lipofilico membrana

italiano

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8,842 Views

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07:31 min

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October 08, 2017

DOI:

10.3791/56273-v

DOI:
10.3791/56273-v

•

07:31 min
October 08, 2017

•

2 Views
, , , ,
1Cellular Biomedicine Group, China, 2Cellular Biomedicine Group, California, 3Department of Orthopedics,Shanghai TCM-Integrated Hospital, 4Department of Orthopedics, Shanghai East Hospital,Tongji University

Summary

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Questo protocollo descrive un modo efficiente per monitorare la persistenza delle cellule e la biodistribuzione di adiposo-derivate cellule staminali mesenchimali umane (haMSCs) di fluorescenza da' etichettatura in un modello di osteoartrite (KOA) del ginocchio di ratto tramite iniezione intra-articolare (IA).

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Cite this Article

Li, M., Hao, M., Jiang, D., Chen, Y., Wang, W. In Vivo Tracking of Human Adipose-derived Mesenchymal Stem Cells in a Rat Knee Osteoarthritis Model with Fluorescent Lipophilic Membrane Dye. J. Vis. Exp. (128), e56273, doi:10.3791/56273 (2017).

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