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Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells
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Developmental Biology
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JoVE Journal Developmental Biology
Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells

Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells

DOI:
10.3791/55196-v

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14:37 min

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November 01, 2017

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1Department of Internal Medicine, Hematology Division,Washington University in St. Louis

Chapters

  • 00:05Title
  • 00:43Mesoderm Differentiation of hPSCs
  • 05:25Specification of CD34+ Hematopoetic Progenitors
  • 06:50Enzymatic Dissociation of EBs and Hematopoetic Progenitor Isolation
  • 08:23Endothelial-to-hematopoetic Transition
  • 10:18T Cell Assay
  • 11:38Results: Successful Differentiation of hPSCs
  • 14:13Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present human pluripotent stem cell (hPSC) culture protocols, used to differentiate hPSCs into CD34+ hematopoietic progenitors. This method uses stage-specific manipulation of canonical WNT signaling to specify cells exclusively to either the definitive or primitive hematopoietic program.

Tags

Human Pluripotent Stem CellsHematopoietic ProgenitorsDirected DifferentiationPrimitive HematopoiesisDefinitive HematopoiesisStage-specific Signal ManipulationEmbryoid BodiesTrypsin EDTAWash BufferHPSC MediaMultigas Incubator

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